Laser Microsurgery in Fission Yeast
نویسندگان
چکیده
منابع مشابه
Laser Microsurgery in Fission Yeast Role of the Mitotic Spindle Midzone in Anaphase B
INTRODUCTION During anaphase B in mitosis, polymerization and sliding of overlapping spindle microtubules (MTs) contribute to the outward movement the spindle pole bodies (SPBs). To probe the mechanism of spindle elongation, we combine fluorescence microscopy, photobleaching, and laser microsurgery in the fission yeast Schizosaccharomyces pombe. RESULTS We demonstrate that a green laser cuts ...
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Manipulation has been used as a powerful investigation technique since the early history of biology. Every technical advance resulted in more refined instruments that led to the discovery of new phenomena and to the solution of old problems. The invention of laser in 1960 gave birth to what is now called optical manipulation: the use of light to interact with matter. Since then, the tremendous ...
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In fission yeast, we propose that the division plane may be positioned by the position of the premitotic nucleus, perhaps by a signal emanating from the nucleus. Gene products involved in the assembly of the ring and its temporal and spatial controls are beginning to be characterized. Some of these, such as mid1p, may be involved in signals that position the division site. In animal cells, the ...
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ژورنال
عنوان ژورنال: Current Biology
سال: 2004
ISSN: 0960-9822
DOI: 10.1016/j.cub.2004.07.028